Molecular Biology

The FastRNA™ SPIN Kit for Microbes quickly and efficiently isolates high-quality, total RNA from tough-to-lyse bacterial cell cultures and other microbial sources in approximately 15 minutes using a zirconium silicate Lysing Matrix (0.1mm) to lyse bacteria and SPIN columns for the purification process.

Components: Lysing Matrix B (0.1mm zirconium silicate) 50 x 2-mL tubes, FastRNA Lysis Buffer 50 mL, FastRNA Prep Buffer 25 mL, FastRNA Wash Buffer 12 mL, Water (DNase/RNase-Free) 1 mL, FastRNA Spin Column 1 50 each, FastRNA Spin Column 2 50 each, Collection Tubes 2mL 100 each.

The FastRNA™ SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast strains, fungi, and algae in approximately 15 minutes using a specialized Lysing Matrix (Yttria-stabilized zirconium oxide, 0.5mm) for cell lysis and SPIN columns for the purification process.

Components: Lysing Matrix Y (0.5mm Yttria-stabilized ZrO2) 50 x 2-mL tubes, FastRNA Lysis Buffer 50 mL, FastRNA Prep Buffer 25 mL, FastRNA Wash Buffer 12 mL, Water (DNase/RNase-Free) 1 mL, FastRNA Spin Column 1 50 each, FastRNA Spin Column 2 50 each, Collection Tubes 2mL 100.

The FastRNA Win Kit for Plant is designed to isolate and purify high quality total RNA from plant cells and tissues for better RT-PCR results. The use of the FastPrep® instrument combined with unique lysis buffers guarantees an efficient lysis of the starting material and a simultaneous inactivation of endogenous RNases. Genomic DNA is separated from the total RNA by binding to specially optimized mineral carrier particles included in the lysis buffer. The RNA is then mixed with ethanol and transferred to the RNA binding membrane. Total RNA then binds, contaminants are washed away and the pure RNA is eluted. No phenol or chloroform is required. Includes protocol for simultaneous isolation of proteins.